Composite

Part:BBa_K152004:Design

Designed by: David Johnston Monje   Group: iGEM08_Guelph   (2008-10-30)


Promoterless Synthetic Operon of CrtE,B,I,Y


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1941
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1478
    Illegal NgoMIV site found at 1608
    Illegal AgeI site found at 732
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To save time, the cloning was done in parrallel. CrtE was ligated to B, and crtI was ligated to crtY. CrtIY was then cut and ligated to CrtEB to yield CrtEBIY.


Source

This operon is made from biobricks BBa_K152001 - BBa_K152003 and the biobrick BBa_K118005 which was sent to us by iGEM Edinburg.

References