Coding
Part:BBa_K1510929:Design
Designed by: Wang,Ching-Yun Group: iGEM14_NYMU-Taipei (2014-10-09)
Circuit injected in Streptococcus mutans that allows S.m. yield N-Acyl homoserine lactone.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 637
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We have to design PCR primers of the constitutive promoter region of Streptococcus mutans to get the constitutive expression of the LuxI gene.
Source
Constitutive Promoter: Comes from Streptococcus mutans genome. LuxI gene: BBa_C0061 Terminator:BBa_B0015