Coding

Part:BBa_K1510929:Design

Designed by: Wang,Ching-Yun   Group: iGEM14_NYMU-Taipei   (2014-10-09)


Circuit injected in Streptococcus mutans that allows S.m. yield N-Acyl homoserine lactone.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 637
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We have to design PCR primers of the constitutive promoter region of Streptococcus mutans to get the constitutive expression of the LuxI gene.


Source

Constitutive Promoter: Comes from Streptococcus mutans genome. LuxI gene: BBa_C0061 Terminator:BBa_B0015

References