Composite

Part:BBa_K1510105:Design

Designed by: Wei-Tai Chen   Group: iGEM14_NYMU-Taipei   (2014-10-06)


sRNA targets histidine kinase 11 mRNA in S.mutans


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Because we need to put our part into vector PVA838 to shuttle plasmid between E.coli and S.mutans. The restriction site before part is EcoRI and BamHI in the last. In addition, to achieve best efficiency, we also consider the spacer between promoter and coding sequence without RBS(because our final product is RNA)


Source

Veg promoter+HK11 sRNA+MicC scaffold+terminator

References