Composite

Part:BBa_K1510006:Design

Designed by: Wei-Tai Chen   Group: iGEM14_NYMU-Taipei   (2014-10-06)


sRNA targets histidine kinase 11 in S.mutans


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4
    Illegal BamHI site found at 191
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Because we need to put our part into vector PVA838 to shuttle plasmid between E.coli and S.mutans. The restriction site before part is EcoRI and BamHI in the last. In addition, to achieve best efficiency, we also consider the spacer between promoter and coding sequence without RBS(because our final product is RNA)


Source

constitutive promoter(veg promoter) is came from Bacillus subtilis, and MicC scaffold is came from E.coli k-12 MG1655. Other parts are synthesized by primer.


References