Composite

Part:BBa_K150010

Designed by: Pascal Kraemer   Group: iGEM08_Heidelberg   (2008-10-21)

ColicinE9 operon

This colicinE9 operon contains all genes from the pColE9-J plasmid. The colE9 gene codes for the lethal colicinE9 protein. The gene for the colE9 immunity protein is responsible for the immunity of the producing host bacteria to the colicinE9 and is therefore controlled by a constitutive promoter. Expression of the atypical lysis protein leads finally to the lysis of the host bacteria and thereby to the release of the colicinE9. Additionally the pColE9-J plasmid contains a colE5 immunity protein and a canonical lysis protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//collections/probiotics
//collections/probiotics/biocontainment
Parameters
None