Translational_Unit

Part:BBa_K1500014:Design

Designed by: Ryan Beckner   Group: iGEM14_UChicago   (2014-10-17)


paroF->MCherry-MutH(E56A)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1346
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

mutagenized mutH was ligated into the paroF+mCherry-LVA construct plasmid

Source

paroF, mutH are genomic XL1Blue, mCherry is from distribution kit.

References