SdaB protein generator without tRNA + 5 amber stops
This part generates the serine deaminase, SdaB (BBa_K847080), which confers basicity resistance with strong promoter/RBS pair (BBa_BBa_J23038), terminator (BBa_B0010). However, this part will not work without the supP tRNA submitted by Stanford-Brown-Spelman 2014 (BBa_K1499251).
Usage and Biology
Serine catabolism creates buffers in the cytoplasm to help counter the effects of the high pH. This enzyme will break down serine to confer resistance to higher pH. Please refer to Stanford-Brown 2012 iGEM's page on this enzyme's part, BBa_K847080.
This part is to be used in the Stanford-Brown-Spelman 2014 iGEM amberless system with the supP tRNA to confer resistance to higher pH. The stop codons inhibit the expression of the complete product, except in amberless cells, that do not have the UAG release factors.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 6
Illegal NheI site found at 29
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 226
Illegal AgeI site found at 549
- 1000Illegal SapI.rc site found at 520
Illegal SapI.rc site found at 667
Verification of Part
The part was sequence verified in the pSB1C3 backbone before submission to the registry. Two reads, forward and reverse, were obtained using VF2 and VR (Figure 1).
This part was not investigated, but it could be verified using a basicity resistance assay.