Generator

Part:BBa_K1497017:Design

Designed by: Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen   Group: iGEM14_TU_Darmstadt   (2014-10-07)


Naringenin producing operon under the T7 promoter BBa_I712074


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1158
    Illegal NheI site found at 4685
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1725
    Illegal BglII site found at 4694
    Illegal XhoI site found at 3692
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1831
    Illegal NgoMIV site found at 2663
    Illegal NgoMIV site found at 4665
    Illegal NgoMIV site found at 5241
    Illegal AgeI site found at 1926
    Illegal AgeI site found at 2092
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3121
    Illegal BsaI site found at 4614
    Illegal BsaI.rc site found at 1357
    Illegal BsaI.rc site found at 4096


Design Notes

d


Source

d

References