Regulatory

Part:BBa_K1493805:Design

Designed by: Max van der Ploeg   Group: iGEM14_Wageningen_UR   (2014-10-07)

promoter with CIλ and Tet operator sites


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The design is based on sequences and methods proposed in Elowitz et. al. 2007 The sequences with the desired operator sites where chosen from the sequences in the supplementary data, and put together in silico. The Promoters where than ordered as two primers with a 20 base pair overlap. In this way it could be used for primer extension. The protocol for primer extension can be found [http://openwetware.org/wiki/Knight:Annealing_and_primer_extension_with_Klenow_polymerase here]

Source

The sequences on which this promoter is based can be found in Elowitz et. al. 2007 in the supplementary data.

References

Cox, R. S., 3rd, Surette, M. G., & Elowitz, M. B. (2007). Programming gene expression with combinatorial promoters. Mol Syst Biol, 3, 145. doi: 10.1038/msb4100187