Composite
Part:BBa_K1493560:Design
Designed by: Michiel Herpers Group: iGEM14_Wageningen_UR (2014-10-06)
pRHA+cIλ+pcI/tet+GFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1811
Design Notes
This part was designed to function as a plasmid to characterize the pcI/tet promoter with the [http://2014.igem.org/Team:Wageningen_UR/project/characterization rhamnose mediated characterization].
This characterization method allows to produce different concentrations of repressor at different concentrations of rhamnose.
Source
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