Coding

Part:BBa_K1491027

Designed by: Danielle Peters   Group: iGEM14_Carnegie_Mellon   (2014-09-19)

T7 RNA Polymerase with Dead ER Intein

T7 RNA polymerase with a non-functional version of the ER intein. Used as a control. It contains a 6x His tag.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2464
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2889
    Illegal AgeI site found at 2853
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2364


[edit]
Categories
Parameters
None