Coding

Part:BBa_K1491025

Designed by: Danielle Peters   Group: iGEM14_Carnegie_Mellon   (2014-09-19)

T7 RNA Polymerase with ER Intein

T7 RNA polymerase with estrogen receptor intein embedded. Non functional with intein. When estrogen is introduced to the system, the intein splices out and links pieces of T7 RNA polymerase together, creating the functional version of the protein. It contains a 6x His tag.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2464
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2889
    Illegal AgeI site found at 2853
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2364


[edit]
Categories
Parameters
None