Generator

Part:BBa_K1486044:Design

Designed by: EPFL iGem team 2014   Group: iGEM14_EPF_Lausanne   (2014-10-05)

IFP[1] Mutated


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When fused to another coding sequence, insert a flexible linker (check out our linker BBa_K1486003 for example).

Source

We received Michnick lab IFP1 and IFP2 sequences from [http://www.addgene.org/52899/ addgene]. The sequence was then mutated by us in order to remove a PstI restriction site.

References

Michnick, S., Tchekanda, E., & Sivanesan, D. (2014, April 20). [http://www.nature.com/nmeth/journal/v11/n6/full/nmeth.2934.html An infrared reporter to detect spatiotemporal dynamics of protein-protein interactions.] Nature Methods, 6-6.