Part:BBa_K1486023

Yeast optimized superfolder GFP

Purpose of the Biobrick

This part corresponds to the sequence of the superfolded GFP optimized for yeast cells. We used it as a tag to control the expression of our genes of interest Hog1 and Pbs2. It was extracted by PCR from plasmids we ordered to addgene:Plasmid 44901 pFA6a-link-yoSuperfolderGFP-Kan and Plasmid 44873 pFA6a-link-yoSuperfolderGFP-CaURA3. This construct was also used to evaluate the interaction between hog1 and pbs2 in S.Cerevisae: we split the sequence and fused the N-Terminal part to pbs2 and the C-Terminal part to hog1.

Associated Biobricks

We assembled this part with the yeast resistance genes and the terminator ADH1. We also made a split sfGFP to evaluate the dimerization of hog1 and pbs2 in response to osmotic stress.

• yeast-sfGFP-ADH1-Kan ( https://parts.igem.org/Part:BBa_K1486026)
• yeast-sfGFP-N ( https://parts.igem.org/Part:BBa_K1486028)
• yeast-sfGFP_N-ADH1-Kan ( https://parts.igem.org/Part:BBa_K1486029)
• yeast-sfGFP-ADH1-Ura ( https://parts.igem.org/Part:BBa_K1486032)
• yeast-sfGFP-C( https://parts.igem.org/Part:BBa_K1486034)
• yeast-sfGFP_C-ADH1-Ura( https://parts.igem.org/Part:BBa_K1486035)

References

Improved Blue, Green, and Red Fluorescent Protein Tagging Vectors for S. cerevisiae. Lee et al (PLoS One. 2013 Jul 2;8(7):e67902. doi: 10.1371/journal.pone.0067902. Print 2013. PubMed)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]