Generator

Part:BBa_K1486018:Design

Designed by: EPFL iGem team 2014   Group: iGEM14_EPF_Lausanne   (2014-09-15)


Arabinose promoter + fLuc[1] + fLuc[2]


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1653
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 1357


Design Notes

When designing the part, we took care of adding a stop codon at the end of the N terminal part of the luciferase and a start codon before the C terminus part. Between the two parts of the split is a short random sequence followed by a second RBS.

Source

The split has been made by PCR amplification on the part BBa_K325108 [[1]] from Cambridge 2010 team (EPIC Firefly).

References

Luker KE, Smith MC, Luker GD, Gammon ST, Piwnica-Worms H, Piwnica-Worms D., (August 17, 2004), Kinetics of regulated protein-protein interactions revealed with firefly luciferase complementation imaging in cells and living animals