Generator

Part:BBa_K1486016:Design

Designed by: EPFL iGem team 2014   Group: iGEM14_EPF_Lausanne   (2014-09-15)


fLuc[1]


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 431
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 135


Design Notes

When fused to another coding sequence, add a flexible linker. Be careful to remove the stop codon when fused to the N-Terminus side of the other coding sequence.

Source

This part has been made by PCR amplification on the part BBa_K325108 [[1]] from Cambridge 2010 team (EPIC Firefly).

References

Luker KE, Smith MC, Luker GD, Gammon ST, Piwnica-Worms H, Piwnica-Worms D., (August 17, 2004), Kinetics of regulated protein-protein interactions revealed with firefly luciferase complementation imaging in cells and living animals