Device

Part:BBa_K1484315:Design

Designed by: Salil Bhate   Group: iGEM14_Cambridge-JIC   (2014-10-08)

Output Expression Cassette (HAP1, Modular Cloning Level 1 + PlantSyntax compatible)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 230
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 255
    Illegal BsaI.rc site found at 245


Design Notes

Contains Level 0 CDS acceptor.


Source

Parts assembled by DNA 2.0.

HAP1-VP16 optimised for plant expression donated by J. Haseloff (Patent CA2460632A1)

References