Plasmid_Backbone

Part:BBa_K1480013:Design

Designed by: Roberto Alejandro Valderrama Alfaro   Group: iGEM14_UANL_Mty-Mexico   (2014-10-17)


pSB3C5 + MID TALEN (K1480001) Target site


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2764
    Illegal XbaI site found at 2779
    Illegal SpeI site found at 1
    Illegal PstI site found at 15
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2764
    Illegal NheI site found at 1402
    Illegal SpeI site found at 1
    Illegal PstI site found at 15
    Illegal NotI site found at 8
    Illegal NotI site found at 2770
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2764
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2764
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2764
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2779
    Illegal SpeI site found at 1
    Illegal PstI site found at 15
    Illegal AgeI site found at 993
    Illegal AgeI site found at 1316
    Illegal AgeI site found at 3565
    Illegal AgeI site found at 3677
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 288


Design Notes

Modifications where inserted by PCR on both directions due to TALEN specificity.


Source

Derived from pSB3C5 plasmid

References