Composite

Part:BBa_K1480000:Design

Designed by: Roberto Alejandro Valderrama Alfaro   Group: iGEM14_UANL_Mty-Mexico   (2014-10-08)


Promoter + RBS + TALEN + Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1334
    Illegal NheI site found at 1538
    Illegal NheI site found at 1946
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3083
    Illegal BamHI site found at 2489
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3023
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1202
    Illegal BsaI.rc site found at 854
    Illegal SapI.rc site found at 2503


Design Notes

Due the target sequence is based on a eukaryotic sequence, TALEN can bind to an undesirable site.


Source

Synthetic part based on an eukaryotic gene.

References