DNA
Part:BBa_K1473018:Design
Designed by: Shan, Zhe Group: iGEM14_TCU_Taiwan (2014-10-01)
gRNA for tetR 2
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This gRNA is designed based on basic rules of gRNA for CRISPR: 20bp upstream to PAM site
Source
From tetracycline resistance gene sequence of bacterial plasmid pO26-L.