DNA

Part:BBa_K1473011:Design

Designed by: Shan, Zhe   Group: iGEM14_TCU_Taiwan   (2014-10-01)


gRNA for ampR 5


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This gRNA is designed based on basic rules of gRNA for CRISPR: 20bp upstream to PAM site


Source

From ampicillin resistance gene sequence of bacterial plasmid pBluescriptII SK(-).

References