Plasmid

Part:BBa_K1473000:Design

Designed by: Shan, Zhe   Group: iGEM14_TCU_Taiwan   (2014-09-10)


pBluescript + RFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 701
    Illegal XbaI site found at 1654
    Illegal SpeI site found at 725
    Illegal PstI site found at 707
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 701
    Illegal SpeI site found at 725
    Illegal PstI site found at 707
    Illegal NotI site found at 1660
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 701
    Illegal BamHI site found at 719
    Illegal XhoI site found at 668
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 701
    Illegal XbaI site found at 1654
    Illegal SpeI site found at 725
    Illegal PstI site found at 707
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 701
    Illegal XbaI site found at 1654
    Illegal SpeI site found at 725
    Illegal PstI site found at 707
    Illegal NgoMIV site found at 129
    Illegal AgeI site found at 1365
    Illegal AgeI site found at 1477
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3036
    Illegal SapI site found at 1953


Design Notes

No


Source

We get pBluescript form our professor. Helper phage M13KO7 is got by ordering.

References