Generator

Part:BBa_K1472611:Design

Designed by: Choi hiu Ying, LEE Cheuk Lam, Tse Yui Shing, Yeung Sin Yu   Group: iGEM14_CityU_HK   (2014-09-28)


IPTG inducible expression of ∆9, ∆12, and ∆15 desaturases


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1278
    Illegal BamHI site found at 673
    Illegal BamHI site found at 1638
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 497
    Illegal AgeI site found at 1459
    Illegal AgeI site found at 1723
    Illegal AgeI site found at 1861
    Illegal AgeI site found at 2694
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The size of the whole device could be relatively large. The selection of promoter is important as it controls when the machine starts producing desaturases. The investment of producing 3 enzymes in a time could be a energy burden for E. coli.


Source

The amino acid sequences of desaturases genes are derived from Synechocystis sp., but codon optimised for expression in E. coli.

References