Device

Part:BBa_K1471012:Design

Designed by: Jaime Antonio del Castillo Nuñez   Group: iGEM14_BIOSINT_Mexico   (2014-11-02)


RBS - MerE - RBS - MerB - RBS - GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 428
    Illegal AgeI site found at 375
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1511


Design Notes

The entire system was ensambled with 3A assembly and including two genes from the mer operon and also a GFP reporter which was used to measure the expression of the system.

Source

Mer genes comes from bacterial genomic sequences

References