Composite

Part:BBa_K1471002:Design

Designed by: Juan Noe Hernandez Salazar   Group: iGEM14_BIOSINT_Mexico   (2014-10-08)

RBS with MerE.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We have to optimized its codons in Arabidopsis Thaliana and removed the restriction sites for EcoR1, Xba1, Spe1 and Pst1.

Source

The RBS came from an Eukaryotic cell and MerE came from bacterial operon mer for mercury resistance.