Part:BBa_K1467103:Experience

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Applications of BBa_K1467103

NRP-UEA iGEM 2014

This promoter was used by iGEM14_NRP-UEA-Norwich to induce expression of several reporter genes. As Methyl Jasmonate is a compound that is known to be produced by plants as a response to pathogen infection, the promoter was used to test our proof on concept system in which various reporters (GFP, Bax, Nbhb1, AmilCP and AmilGFP) were transcribed by activation of PDF1.2 in the presence of methyl jasmonate but not when methyl jasmonate was not present, showing that the promoter functions as expected. Evidence of this using the GFP reporter is shown below.

Figure 1: Nicotiana benthamiana leaves under UV light following infiltration (red circles) with Agrobacterium tumefaciens carrying plasmids encoding PDF1.2_GFP_ocs. Leaf (B) has been treated with Methyl Jasmonate whilst the control, leaf (A), was untreated.

There is GFP expression on the leaf from the plant treated with a methyl jasmonate spray, showing that the promoter is active (B). On the plant not treated with methyl jasmonate, there is no expression of GFP showing that the PDF1.2 promoter is not active (A). This shows how the promoter is induced in the presence of methyl jasmonate.

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