Regulatory

Part:BBa_K1467101:Design

Designed by: Cara Deal   Group: iGEM14_NRP-UEA-Norwich   (2014-09-24)


GoldenGate compatible 35s Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 392
    Illegal BglII site found at 1138
    Illegal XhoI site found at 1342
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Domestication for GoldenGate (Removal of internal BsaI and BpiI recognition sequences).


Source

Cauliflower Mosaic Virus (CaMV)

References