Part:BBa_K1467100:Experience

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Applications of BBa_K1467100

iGEM14_NRP-UEA-Norwich have used this part to convert GoldenGate "Pro + 5U" (promoter and 5' untranslated leader) modules from the GoldenGate MoClo Assembly Standard into standard BioBricks. The cloning reaction was a Golden Gate one-step, one-pot reaction where the restriction enzyme, ligase and (intact, uncut) donating and recipient plasmids were all incubated together in a single reaction before transformation into competent E.coli cells. Cloning was very successful as the vast majority of the colonies are white as the RFP sequence was replaced by the new part.

User Reviews

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BBa_K1467100 Valencia_UPV 2104 team

Our team succesfully used this part to send Part:BBa_K1554000 to the Registry of Parts. We report its high efficiency and its ease of use.

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