Translational_Unit

Part:BBa_K1465303:Design

Designed by: iGEM-Team Bielefeld 2014   Group: iGEM14_Bielefeld-CeBiTec   (2014-10-08)

Isobutanol pathway with adhA from Lactococcus lactis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7182
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5669
    Illegal XhoI site found at 4949
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2332
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1918
    Illegal BsaI site found at 4735
    Illegal BsaI site found at 4992
    Illegal BsaI.rc site found at 320
    Illegal BsaI.rc site found at 914
    Illegal BsaI.rc site found at 2737


Design Notes

We used Gibson as the cloning method for the part BBa_K1465302. Afterwards we did several BioBrick Assemblies for combining the parts BBa_K1465302 and BBa_K1465301.

Source