Device

Part:BBa_K1465226:Design

Designed by: iGEM-Team Bielefeld 2014   Group: iGEM14_Bielefeld-CeBiTec   (2014-10-07)


sRNA of phosphofructokinase A under the control of ptac promoter (ptac_sRNA:pfkA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1351
    Illegal XhoI site found at 1357
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Classical [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#BioBrick Suffix Insertion] of the sRNA:pfkA (BBa_K1465225) into the pSB1C3 backbone of the Ptac promotor containing also the constitutive lacI repressor (BBa_K731500).

Source

BBa_K1465225: sRNA:pfkA
BBa_K731500: [LacI + LacIq promoter] reverse + [tac promoter + lac operator] forward

References