Device

Part:BBa_K1465214

Designed by: iGEM-Team Bielefeld 2014   Group: iGEM14_Bielefeld-CeBiTec   (2014-10-09)

RubisCO of Halothiobacillus neapolitanus and phosphoribulokinase A (prkA) under control of ptac


The RuBisCO of Halothiobacillus neapolitanus is an enzyme which can catalyze the reaction of ribulose 1,5-bisphosphate to 3-phosphoglycerate. In this step CO2 is bound. The phosphoribulokinase A (PrkA) catalyzes the reaction before, where ribulose 5-phosphate is converted to ribulose 1,5-bisphosphate. The two enzymes are characterized here BBa_K1465213 (RuBisCO) and here BBa_K1465201 (prkA).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2613
    Illegal NheI site found at 4055
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2056
    Illegal BamHI site found at 2737
    Illegal BamHI site found at 3244
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3703
    Illegal AgeI site found at 3974
    Illegal AgeI site found at 4045
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None