Composite
Part:BBa_K1465209:Design
Designed by: iGEM-Team Bielefeld 2014 Group: iGEM14_Bielefeld-CeBiTec (2014-10-02)
Carbonic anhydrase (csoS3), shell-proteins (csoS14) and pore protein (csoS1D) of the carboxysome
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 166
Illegal AgeI site found at 2277 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Classical [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#BioBrick Suffix Insertion] of the shell-proteins and the carbonic anhydrase (BBa_K1465208) into the pSB1C3 backbone of the pore-forming protein (BBa_K1465207).
Source
BBa_K1465208: shell-proteins (BBa_K1465206) and carbonic anhydrase (BBa_K1465205) from Halothiobacillus neapolitanus.
BBa_K1465207: pore-forming protein csoS1D from Halothiobacillus neapolitanus.