DNA
spacer

Part:BBa_K1463030:Design

Designed by: Beth Greig   Group: iGEM14_Glasgow   (2014-08-14)


Switch Nucleotide Spacer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Created this part with 2 restriction sites when combined with scar sites in larger composite part.

Restriction sites in switch - HindIII
HindIII was designed into the switch downstream of the promoter. This allowed for confirmation of the "flip" of the switch by restriction digest.
Using both HindIII and PstI produces different sized fragments dependent on the orientation of the switch promoter.

Source

Synthesised by IDT Technologies from a given sequence. Sequence is not from genome.

References