Composite

Part:BBa_K1462570

Designed by: zhanru Chen   Group: iGEM14_SCUT   (2014-10-14)

GAL1+GBD+SH3+SH3+PDZ+Tom22+ADH1

Scaffold protein, with a leading peptide Tom22, can locate on the outer mitochondria membrane with the scale of GBD: SH3: PDZ==1:2:1.We built up the scale to affirm the best scale. In this way , we tried to fix the PRK, RuBisCO, CA to a limited space to make full use of ATP and CO2 so that we coule improve the pyruvate offering to the butanol pathway.


Figure 1.This is the pathway and the structure of the carbon capture. As we see, with the help of ATP,the RuBiSco-5P change into RuBiSco-1,5P by PRK, and then change into 3P-glyrerate by RuBiSco, and CA can help us to adjust the concentration of carbon dioxide. The x,y,z are the number of scaffold unit.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1759
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1759
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 615
    Illegal BamHI site found at 648
    Illegal BamHI site found at 820
    Illegal BamHI site found at 1029
    Illegal BamHI site found at 1238
    Illegal BamHI site found at 1561
    Illegal XhoI site found at 801
    Illegal XhoI site found at 1010
    Illegal XhoI site found at 1219
    Illegal XhoI site found at 1542
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1759
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1759
    Illegal AgeI site found at 150
  • 1000
    COMPATIBLE WITH RFC[1000]


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