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Regulatory

Part:BBa_K1461107:Design

Designed by: Shunsuke Sumi   Group: iGEM14_UT-Tokyo   (2014-09-23)


EGP-2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

EPCAM is expressed in CTC. Therefore, EGP-2 is considered to be a good candidate for a marker.


Source

1. McLaughlin, P. M., Trzpis, M., Kroesen, B. J., Helfrich, W., Terpstra, P., Dokter, W. H., ... & Harmsen, M. C. (2004). Use of the EGP-2/Ep-CAM promoter for targeted expression of heterologous genes in carcinoma derived cell lines. Cancer gene therapy, 11(9), 603-612. 2.Danda, R., Krishnan, G., Ganapathy, K., Krishnan, U. M., Vikas, K., Elchuri, S., ... & Krishnakumar, S. (2013). Targeted Expression of Suicide Gene by Tissue-Specific Promoter and MicroRNA Regulation for Cancer Gene Therapy. PloS one, 8(12), e83398.

References