Composite
Part:BBa_K1460004:Experience
Designed by: Eric Holmes Group: iGEM14_Cornell (2014-08-26)
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Applications of BBa_K1460004
Cornell 2014 Results
Cells successfully expressing merT and merP should be transporting more mercury ions past the cell wall. This would lead to increased mercury sensitivity. To test for mercury sensitivity, E.coli BL21 and engineered BL21 with part BBa_K1460004 in the cmr plasmid pSB1C3 were grown for a 24 hour period in LB with .5 uM Hg (a mercury concentration we found to have moderate toxicity to wild type BL21 cells.
What we observe from this above chart is what we expect. We see that BL21 engineered with BBa_K1460004 has impaired growth when compared to wild type BL21. This suggests that, in fact, BBa_K1460004 acts as expected and engineered cells successfully transport more mercury ions past the membrane than wild type cells.