Coding

Part:BBa_K1456006:Design

Designed by: Mustafa Semih Elitok   Group: iGEM14_ATOMS-Turkiye   (2014-07-21)

Aprotinin: Oxygen Radical Inhibitor

ATOMS-ODD3.png

Cloning

  • Aprotinin and pTRE vector was digested using EcoRI ve BamHI enzymes following by their ligation which cloned aprotinin into the pTRE vector. The isolated plasmid from the bacteria was then cut-checked using EcoRI ve BamHI enzymes and the following results were achieved:


ATOMS Aprotinin Results2.png



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 5
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 27
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

We ordered this part to be synthesized. Sequence originally belongs to Bos taurus (bovine)


References