Coding

Part:BBa_K1453500:Design

Designed by: Binhan Hao   Group: iGEM14_SJTU-BioX-Shanghai   (2014-10-16)


New TAL_left_right


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 27
    Illegal NotI site found at 489
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
    Illegal BamHI site found at 463
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It has the best sticky ends you can find on the sequence. BsmBI can digest these parts and get a 4bp sticky end at last. You can use golden gate method to ligate 7 parts of New TAL and get a whole TALE.


Source

The parts are synthesized by biological company.

References