Composite

Part:BBa_K1446002:Design

Designed by: Philipp Lorenz   Group: iGEM14_Oxford   (2014-09-29)


superfolder GFP with N-terminal pdu-microcompartment tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 91


Design Notes

We have designed Primers to amplify sfGFP from a plasmid, provided by Dr. Ciaran Kelly, in order to attach the microcompartment tag upstream of the sfGFP-coding region.


Source

We received a sample of sfGFP in a holding vector, provided by Dr. Ciaran Kelly.

References