Plasmid_Backbone

Part:BBa_K1445002:Design

Designed by: Josephina Hendrix   Group: iGEM14_CU-Boulder   (2014-09-25)

High copy BioBricked M13 phagemid vector


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2990
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2996
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2990
    Illegal BglII site found at 2852
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2990
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2990
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3005
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1240


Design Notes

Plasmid was designed to have the iGEM VF2 and VR sequencing sites along with translational and transcriptional terminators flanking the MCS. There are two amino acid changes from the published Litmus28i sequence located in the ampicillin resistance gene but these changes do not cause loss of function.


Source

Limtus28i phagemid vector from NEB. Areas flanking MCS were cloned from pSB3C5 backbone.

References