Plasmid_Backbone

Part:BBa_K1444027:Design

Designed by: Dave Curran   Group: iGEM14_Calgary   (2014-10-17)

pUC57 cloning vector from BioBasic


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 1727
    Illegal SapI site found at 644


Design Notes

This plasmid contains ampicillin resistance, blue/white cloning selection, and is a high copy-number vector.


Source

This cloning vector came from the gene synthesis services offered by [http://store.biobasic.com/gene-synthesis/ BioBasic].

References