RNA

Part:BBa_K1442113:Design

Designed by: Becky Seeley   Group: iGEM14_Warwick   (2014-10-11)

siRNA for DPP-IV


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequences surrounding the siRNA were designed so that in the positive sense there are no double stranded regions long enough to be recognised by DICER. This is gone by there being a lot of GU pairings in the negative sense so the hairpin that DICER recognises is present but in the positive sense the two regions are not complementary.


Source

The sequence was obtained from the paper Inamoto et al, 2007 who used TAKARA BIO to design the siRNA.

References

Inamoto et al (2007). Humanised anti CD-26 monoclonal antibody as a treatment for Malignant Mesothelioma Tumours. Clinical cancer research; volume 13. 4191-4200