Primer

Part:BBa_K1441015:Design

Designed by: Matthew Brewer   Group: iGEM14_Georgia_State   (2014-10-10)


DNA ligase Ecor1 F primer


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 7
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 7
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 7
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 7
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 7
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primers specific for the alteration of IGEM prefixes. The melting temperatures and annealing temperatures of the primers had to be taken into consideration when designing the primers.


Source

Primers were designed from the DNA ligase kit plate part (BBa_K525710).

References