Coding

Part:BBa_K144000:Design

Designed by: Caleb Dulaney   Group: iGEM08_Mississippi_State   (2008-10-27)


Lignin Peroxidase A


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 620
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 620
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 583
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 620
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 620
    Illegal NgoMIV site found at 565
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 508
    Illegal BsaI.rc site found at 1039


Design Notes

This part is one of a family of highly homologous genes in P.C. It was nearly impossible to isolate the gene using primers specific for the coding sequence. It was necessary to use a sequential set of PCR primers specific for the upstream and downstream regions and then the specific coding sequence, respectively.


Source

cDNA of Phanerochaete Chrysosporium grown on Aspen Wood Culture at University of Wisconsin/USDA Forest Products Lab.


References