Part:BBa_K1439001:Design

This part contains a reporter gene BBa_J04450, combined with OriTRP4. Used to test plasmid mobility.

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 20
  Illegal NgoMIV site found at 30
  Illegal AgeI site found at 1139
  Illegal AgeI site found at 1251
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

For checking conjugation

Source

OriTRP4 comes form the RP4 plasmid, from 51098bp to 51448bp, reporter gene comes from BBa_J04450.