Plasmid_Backbone

Part:BBa_K1438032:Design

Designed by: Johann Bauerfeind   Group: iGEM14_Berlin   (2014-10-10)


PJS418


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Illegal suffix found at 2267
    Illegal XbaI site found at 1383
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SpeI site found at 2268
    Illegal PstI site found at 2282
    Illegal NotI site found at 2275
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BglII site found at 1495
    Illegal BamHI site found at 2366
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Illegal suffix found at 2268
    Illegal XbaI site found at 1383
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Illegal suffix found at 2258
    Illegal XbaI site found at 1383
    Illegal NgoMIV site found at 1391
    Illegal NgoMIV site found at 2499
    Illegal AgeI site found at 3833
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 2115


Design Notes

pJS418


Source

pJS418

References