Composite

Part:BBa_K1435002:Design

Designed by: Matthew Chan   Group: iGEM14_Queens_Canada   (2014-10-17)


N-terminal GFP w/ an N-terminal Intein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 609
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A


Source

This part was created using de novo DNA synthesis. The intein sequence was obtained via InBase, a peer-reviewed database of inteins. The intein sequence was isolated from Cyanobacterium synechocystis.

References