Coding
DOUBLE

Part:BBa_K1433020:Design

Designed by: Chaofan Zhang   Group: iGEM14_ZJU-China   (2014-10-09)


P-A-T-T-B-C-T-T-D-B0034-GFP-T


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1393


Design Notes

no


Source

Derive from 2014 Distribution Kit and synthesis by Genewiz.

References

  1. Baba T, Ara T, Hasegawa M, et al. Construction of Escherichia coli K‐12 in‐frame, single‐gene knockout mutants: the Keio collection[J]. Molecular systems biology, 2006, 2(1).
  2. Mosberg J A, Lajoie M J, Church G M. Lambda red recombineering in Escherichia coli occurs through a fully single-stranded intermediate[J]. Genetics, 2010, 186(3): 791-799.
  3. Datsenko K A, Wanner B L. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products[J]. Proceedings of the National Academy of Sciences, 2000, 97(12): 6640-6645.
    4. li>Bonnet J, Subsoontorn P, Endy D. Rewritable digital data storage in live cells via engineered control of recombination directionality[J]. Proceedings of the National Academy of Sciences, 2012, 109(23): 8884-8889.
  4. Siuti P, Yazbek J, Lu T K. Synthetic circuits integrating logic and memory in living cells[J]. Nature biotechnology, 2013, 31(5): 448-452.
  5. Sharan S K, Thomason L C, Kuznetsov S G, et al. Recombineering: a homologous recombination-based method of genetic engineering[J]. Nature protocols, 2009, 4(2): 206-223.