Coding
SET

Part:BBa_K1433011:Design

Designed by: Chaofan Zhang   Group: iGEM14_ZJU-China   (2014-10-09)


Terminator-RFP-RBS-attB-P-attP-RBS-GFP-Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 933
    Illegal NheI site found at 956
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 880
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 900
    Illegal BsaI.rc site found at 983
    Illegal BsaI.rc site found at 1684


Design Notes

no


Source

Derive from 2014 Distribution Kit.

References

  1. Bonnet J, Subsoontorn P, Endy D. Rewritable digital data storage in live cells via engineered control of recombination directionality[J]. Proceedings of the National Academy of Sciences, 2012, 109(23): 8884-8889.
  2. Siuti P, Yazbek J, Lu T K. Synthetic circuits integrating logic and memory in living cells[J]. Nature biotechnology, 2013, 31(5): 448-452.