Coding

Part:BBa_K1433004

Designed by: Hanqing Liu   Group: iGEM14_ZJU-China   (2014-09-28)

gp47 CDS

Bxb1 gp47 is an excisionase and Bxb1 gp35 is a serine integrase in Mycobacterium phage Bxb1. This intergrase along can typically catalyzes site-specific recombination and mediate DNA inversion from an BP to LR state. Heterologous expression of Bxb1 integrase gp35 and excisionase gp47 could mediate DNA restore from an LR to BP state.

ZJU_int-xis-bplr.gif

This part is the coding sequence of Bxb1 excisionase gp47.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 772
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None